A reverse phase stability indicating HPLC method for simultaneous determination of two antispasmodic drugs in pharmaceutical\nparenteral dosage forms (injectable) and in serum has been developed and validated. Mobile phase ingredients consist of\nAcetonitrile : buffer : sulfuric acid 0.1M (50 : 50 : 0.3 v/v/v), at flow rate 1.0 mL/min using a Hibar ????Bondapak ODS C18 column\nmonitored at dual wavelength of 266nm and 205nm for phloroglucinol and trimethylphloroglucinol, respectively. The drugs\nwere subjected to stress conditions of hydrolysis (oxidation, base, acid, and thermal degradation). Oxidation degraded the\nmolecule drastically while there was not so much significant effect of other stress conditions. The calibration curve was linear\nwith a correlation coefficient of 0.9999 and 0.9992 for PG and TMP, respectively. The drug recoveries fall in the range of\n98.56% and 101.24% with 10 pg/mL and 33 pg/mL limit of detection and limit of quantification for both phloroglucinol and\ntrimethylphloroglucinol. The method was validated in accordance with ICH guidelines and was applied successfully to quantify\nthe amount of trimethylphloroglucinol and phloroglucinol in bulk, injectable form and physiological fluid. Forced degradation\nstudies proved the stability indicating abilities of the method.
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